• Micropipetting and centrifuges
• Metric Conversions and Scientific measures

• Plasmid Purification by Alkaline lysis
• Agarose Gel Electrophoresis
• Plasmid Identification using restriction enzymes
• In silico Digestion

• Bacterial transformation: heat shock
• History of DNA as genetic material
• pGlo/Genotype to Phenotype → Hypothesis test/Predictions

• Plasmid Structure → Visualize transformation predictions
• Transcriptional Control

Alberts 331-333

• DNA isolation from Eukaryotes
• Introduction to DNA replication and PCR
• Genetics Review

• VNTR, STRs, RFLP
• PTC Paper and human alleles (SNP)

• Mitochondrial Haplotyping (activity)
• Using Transposons for lineage with Alu insertion

• Quiz 2
• Barcoding of Life (COI and RbcL activity)
• Tissue Culture demonstration

• Gene Expression:
• Prokaryotic transcription
• Eukaryotic mRNA
• RNA isolation and Reverse Transcription

• Quantitative Real-Time PCR
• Sequencing technology (Sanger)

• Quiz 3
• Bioinformatics: Analysis of mitochondrial DNA and Barcoding
• Primer Design for qPCR

• Illumina
• Ion Torrent
• PacBio
• MinION
• qPCR results review

• Transgenic Technology and activity
• Transfection of Eukaryotic cells (demonstration)

• Introducing biotechnology: Protein Production
• Protein Purification (activity)
• Review Transfection