1

• Micropipetting and centrifuges
• Metric Conversions and Scientific measures

2

• Plasmid Purification by Alkaline lysis
• Agarose Gel Electrophoresis
• Plasmid Identification using restriction enzymes
• In silico Digestion

3

• Bacterial transformation: heat shock
• History of DNA as genetic material
• pGlo/Genotype to Phenotype → Hypothesis test/Predictions

4

• Plasmid Structure → Visualize transformation predictions
• Gene Expression
  • Prokaryotic Transcription

5

• DNA isolation from Eukaryotes
• Introduction to DNA replication and PCR
• Genetics Review

6

• Crime scene PCR
• VNTR,STRs, RFLP
• PTC Paper and human alleles (SNP)

7

• Tracing Origins
  • Mitochondrial Haplotyping (activity)
  • UsingTransposons for lineage with Alu insertion

8

• Quiz 2
• Barcoding of Life
  • (COI and RbcL activity)
• Tissue Culture demonstration

9

• Introduction to Tissue Culture
• Gene Expression: RNA isolation and Reverse Transcription

10

• Quantitative Real-Time PCR
• Sequencing technology (Sanger)
• Gene Expression Omnibus and GEO2R
  • Querying GEO

11

Quiz 3

• GEO2R and comparing expression
• Bioinformatics
• Primer Design for qPCR

12

• Sequencing technology (Next Generation Sequencing)
  • Illumina
  • Ion Torrent
  • PacBio
  • MinION
  • qPCR results review

13

• Gene Cloning Workflow
  • Transgenic Technology and activity
  • Transfection of Eukaryotic cells (demonstration)
  • CRISPR-Cas9

14

• GFP Protein purification
  • Introducing biotechnology: Insulin as an example
  • Protein Purification(activity)
  • Review Transfection

15