Antibiotic Resistance

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  • Acid-Fast   Uploaded by Maxine Fuchs on April 30, 2018
  • Bacillus Capsules   Uploaded by Ramona on February 23, 2018
    After doing the capsule staining technique, this picture was taken by one of my lecture classmate. Capsules are delicate structures of the cell that can be destroyed very easily by doing other staining techniques, such as Gram stain. My capsules were not visible through the microscope because I made the mistake of washing the smear with H2O.During Capsule staining, the cell and background are stained with crystal violet while the capsules of the cells are unstained.<br />
  • Bacillus subtilus (Endospore Stain)   Uploaded by MariaAschenbrenner on February 26, 2018
    Vegetative cells are described are those that are actively metabolizing and dividing. When exposed to harsh conditions like nutrient limitation, temperature changes and waste accumulation some of these vegetative cells will eventually die. However, bacteria such as Bacillus, Clostridium and Sporogenous produce a structure called Endospores, through the process of sporulation, in order to protect themselves from these hazardious conditions. Endospores are able to withstand extreme heat, drying, radiation, freezing and even chemicals. The Endospore stain is classified as a diferential stain and will help us distinguish vegetative cells from endospores. During this experiment we used the Schaaeffer-Fulton method. First we used the primary stain, Malachite, the to ensure that the endospores are penetrated and dyed we steamed the slides over boiling water for 5 minutes. After this, we decolorized the slide and counterstained with safranin to dye the vegetative cells. At the end of this experiment, Vegetative cells should appear pink while endospores should be green. As you can see in the picture we can see both pink and green, indicating the presence of both vegetative cells and endospores. I am using images provided by my classmates because during this experiment I made a mistake of inadequate heat fixing and using too much water to decolorize slide and was therefore not able to see any cells under the microscope.
  • Bacteria Chart   Uploaded by on May 17, 2018
  • Bacteria chart   Uploaded by MariaAschenbrenner on May 21, 2018
  • bacteria chart .pdf   Uploaded by Mohammed on April 30, 2018
  • bacteria chart .pdf   Uploaded by Mohammed on April 30, 2018
  • Capsule Stain   Uploaded by Maxine Fuchs on April 30, 2018
  • Click link in description! Brain Awareness Extra credit presentation   Uploaded by Maxine Fuchs on May 16, 2018
    https://docs.google.com/presentation/d/1tgVG0DTfA5rQ1UiNVCHSEjdJzTta_kV5jsCyszOq6zY/edit?usp=sharing
  • Endospore stain   Uploaded by Maxine Fuchs on April 30, 2018
  • EnteroPluri Test   Uploaded by sdenis on May 23, 2018
    EnteroPluri test is a 12-sector system containing special culture media that permits identification of the Enterobactericiae and other gram negative, oxidase negative bacteria. Allows the simulataneous inoculation of all media present in the sectors and executions of 15 biochemical reactions. Microorganisms is identified evaluating the color change of the different culture media after 18-26 hours incubation at 37deg Centigrade by a code number obtained from biochemical reactions interpretation.
  • EnteroPluri- Test Opal Thomas   Uploaded by OPAL THOMAS on May 23, 2018
    In a clinical setting where expedience, efficency accuracy and time are all important factor for diagnosing a pathogen; a multi test system solves all those problem. EnteroPluri -Test system is a single- piece unit that is able to take /run 10- 100 test simultaneously. The 12 compartment tube contains in each different medias, several of the compartment are covered with wax to create anaerobic condition. The objective is to generate identification of an organism rather than focus specifically on the individual reaction in each compartment. After inoculating each tube by drawing the wire while rotating it; after resealing by putting the caps on; later holes were punched through to create aerobic conditions, and incubated for 24hrs at 37 degrees Celius. Starting from the blue cap: first agar is 1. GLUCOSE positive sugar fermentation is yellow which is usually accompanied by gas indicated by bubbles and gaps in the agar. 2-3. ORNITHINE and LYSINE, [lysine is encoded by the codons AAA and AAG, is an α-amino acid that is used in the biosynthesis of proteins]. A purple color indicates a positive reaction [decarboxylation]. 4. Hydrogen sulfide [H2S]/INDOLE compartment will read two results. Black color is positive for H2S production, with the reagent added; positive for indole from tryptophan turns the reagent bright red.<br /> 5-8. ADONITOL, LACTOSE, ARABINOSE and SORBITAL; yellow color is a positive indication fer fermentation of sugars. 9. VOGES-PROSKAUER confirms identification .[ not generating an ID code, results are not red]. 10. DULCITOL/PHENLALANINE compartments yellow indicates positive fermentation of dilcitol [sugar]. Black/smokey gray color is psitive for phenylalnamine [deamination]. 11. UREA compartment, pink indicate spositive for urease. 13. CITRATE; blue indicates utilization of citrate [sugar] as a carbon source. Additionally each compartment has a number assigned, and grouped 1-5. Each grouped only the positive numbers are tallied. then combined all the groups number are added to identify the organism [ using a KEY provided].
  • EnteroPluri-Test (12-sector system)   Uploaded by Maxine Fuchs on May 25, 2018
    P.V. & B.T. & F.O.M.A
  • EnteroPluri-Test (code)   Uploaded by Maxine Fuchs on May 25, 2018
  • Extra Credit: Brain and Health awareness day   Uploaded by on May 17, 2018
  • Extra Credit: Stress vs. Genetic mutations   Uploaded by on May 17, 2018
  • FACTS about AR   Uploaded by Nasreen Haque on February 7, 2018
    This will provide you background information on AR.
  • Gram Stain   Uploaded by Maxine Fuchs on April 30, 2018
  • Identification of unknown bacteria #17   Uploaded by MariaAschenbrenner on May 21, 2018
  • Identification of Unknown Bacteria in Test Tube 3   Uploaded by Afua W on May 17, 2018
  • Idetificantion of Bacteria # 11   Uploaded by Kelly on May 17, 2018
  • Initial Results & Most Useful Additional Tests to Perform   Uploaded by Maxine Fuchs on April 30, 2018
  • Lab Practical #1   Uploaded by on March 14, 2018
  • Lab practical #1 Maria Alba   Uploaded by MariaAschenbrenner on March 14, 2018
  • Lab practical #1 Maria Alba   Uploaded by MariaAschenbrenner on March 15, 2018
  • Lab Practical 1   Uploaded by Afua W on March 14, 2018
    Identification of unknown bacterium in Tube 3
  • Lab Practical: Identification of unknown bacteria #10   Uploaded by on May 17, 2018
  • Maxine Fuchs   Uploaded by Maxine Fuchs on February 26, 2018
    Nutrient agar
  • Maxine Fuchs   Uploaded by Maxine Fuchs on February 26, 2018
    Lab 6 - Endospores
  • Maxine Fuchs   Uploaded by Maxine Fuchs on March 15, 2018
    Unknown #20 Bacteria
  • Negative Stain   Uploaded by Maxine Fuchs on April 30, 2018
  • Phantom Limb Syndrome (Extra Credit)   Uploaded by Kelly on May 23, 2018
  • Practical #1 Identifying unknown bacterium   Uploaded by Solange Sylvester on March 14, 2018
  • Practical #2: Identification of unknown in tube 5   Uploaded by asheleebarreau on May 21, 2018
  • Review Weeks 1-5   Uploaded by Mohammed on March 4, 2018
  • Review Weeks 6-8   Uploaded by Mohammed on April 15, 2018
    Cannot upload the file, so here is the link for the review. Its an open link so that you dont have to download anything<br /> <br /> https://drive.google.com/file/d/1cGXeBGWAMpS2nxue_zzQDxQw6eqb3XM9/view?usp=sharing
  • Simple stain   Uploaded by Maxine Fuchs on April 30, 2018
  • Staphylococcus aureus (Endospore)   Uploaded by diegosr96 on February 25, 2018
    I have uploaded a photo of Staphylococcus aureus that was handed to me by a classmate after the Endospore lab was done. Endospores are formed by bacterias in order to survive hostile conditions like heat, desiccation, chemicals and radiation. As you can see in the photo there is a mixture of both dual-stained pink and green, the Malachite Green stains both vegetative and endospores but when heat is applied and water is used after the green will stay on Endospores only. Sefranin is then applied to counterstain the the cells and vegetative cells will be PINK and Endospores will be GREEN. When I tried to do this procedure I had placed too much water which caused me to wash away some of the dye which is why I am using a class mates photo for the analysis.
  • Template_Identification of Unknown Bacteria   Uploaded by Maxine Fuchs on April 30, 2018
  • Unknown Bacteria determination #23 : E. Coli   Uploaded by Mohammed on March 14, 2018
  • Unknown Bacteria Identification #6 - Practical #2   Uploaded by Solange Sylvester on May 16, 2018
    Final Lab Practical
  • Unknown Bacteria Practical Review   Uploaded by Mohammed on March 4, 2018
    Breakdown of the bacterias we have used so far in class, and what test can be used to identify which. Please pm me if you guys see any discrepancies, or if there is something that can be added to the flowchart. The more tests we can do, the better chances of identifying the bacteria. Good luck everyone.