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Gram Stain
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Capsule Stain
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Negative Stain
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Template_Identification of Unknown Bacteria
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Initial Results & Most Useful Additional Tests to Perform
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bacteria chart .pdf
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Review Weeks 6-8
Description: Cannot upload the file, so here is the link for the review. Its an open link so that you dont have to download anything<br /> <br /> https://drive.google.com/file/d/1cGXeBGWAMpS2nxue_zzQDxQw6eqb3XM9/view?usp=sharing
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Maxine Fuchs
Description: Unknown #20 Bacteria
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Lab practical #1 Maria Alba
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Lab practical #1 Maria Alba
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Lab Practical #1
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Practical #1 Identifying unknown bacterium
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Lab Practical 1
Description: Identification of unknown bacterium in Tube 3
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Unknown Bacteria determination #23 : E. Coli
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Review Weeks 1-5
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Unknown Bacteria Practical Review
Description: Breakdown of the bacterias we have used so far in class, and what test can be used to identify which. Please pm me if you guys see any discrepancies, or if there is something that can be added to the flowchart. The more tests we can do, the better chances of identifying the bacteria. Good luck everyone.
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Maxine Fuchs
Description: Nutrient agar
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Maxine Fuchs
Description: Lab 6 - Endospores
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Bacillus subtilus (Endospore Stain)
Description: Vegetative cells are described are those that are actively metabolizing and dividing. When exposed to harsh conditions like nutrient limitation, temperature changes and waste accumulation some of these vegetative cells will eventually die. However, bacteria such as Bacillus, Clostridium and Sporogenous produce a structure called Endospores, through the process of sporulation, in order to protect themselves from these hazardious conditions. Endospores are able to withstand extreme heat, drying, radiation, freezing and even chemicals. The Endospore stain is classified as a diferential stain and will help us distinguish vegetative cells from endospores. During this experiment we used the Schaaeffer-Fulton method. First we used the primary stain, Malachite, the to ensure that the endospores are penetrated and dyed we steamed the slides over boiling water for 5 minutes. After this, we decolorized the slide and counterstained with safranin to dye the vegetative cells. At the end of this experiment, Vegetative cells should appear pink while endospores should be green. As you can see in the picture we can see both pink and green, indicating the presence of both vegetative cells and endospores. I am using images provided by my classmates because during this experiment I made a mistake of inadequate heat fixing and using too much water to decolorize slide and was therefore not able to see any cells under the microscope.
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Staphylococcus aureus (Endospore)
Description: I have uploaded a photo of Staphylococcus aureus that was handed to me by a classmate after the Endospore lab was done. Endospores are formed by bacterias in order to survive hostile conditions like heat, desiccation, chemicals and radiation. As you can see in the photo there is a mixture of both dual-stained pink and green, the Malachite Green stains both vegetative and endospores but when heat is applied and water is used after the green will stay on Endospores only. Sefranin is then applied to counterstain the the cells and vegetative cells will be PINK and Endospores will be GREEN. When I tried to do this procedure I had placed too much water which caused me to wash away some of the dye which is why I am using a class mates photo for the analysis.
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