Lab 9 Outline

Background Information

• https://openlab.citytech.cuny.edu/bio1101coursehub/lessons/lab/cellular-respiration/
• https://openlab.citytech.cuny.edu/bio1101coursehub/lessons/lab/fermentation/

Activities

• https://openlab.citytech.cuny.edu/bio1101coursehub/lessons/lab/lab-9-activities/

General Outline

1. Set-up the fermentation tubes as soon as possible.
   1. Dissolve one packet of yeast in 120 ml of distilled H₂O
   2. Have four groups setting up two of the fermentation conditions. You should coordinate with the lactose group to set up condition 9 with the lactase pill.
   3. Review the conditions of the experiment and ask students to hypothesize the amount of activity based on the conditions (Process of Science)
2. The experiment is very passive and provides you ample time to perform a recitation about the following:
   1. Chemical bonds as a storage of energy
      1. Please note the demonstration of triboluminescence in the video
         https://youtu.be/6DPNeSajakI which runs 11 seconds. (Integration of Knowledge, Transformation of Energy)
      2. Electrons of the bonds and the electrons harvested throughout respiration and subsequent flux through the electron transport chain
   2. Glycolysis
   3. Fermentation
   4. Preparatory Reaction
   5. Oxidative Phosphorylation
   6. The origin of mitochondria and other details of this system (Evolution)
      1. Anatomy of mitochondria: cristae for surface area, double membrane (Structure and Function)
3. Review the results of the fermentation experiment

Curricular Notes

These exercises emphasize NSF Vision & Change Core Competencies:

1. Process of Science
2. Integration of Knowledge

This unit emphasizes the following NSF Vision & Change Core Concepts:

1. Evolution
2. Structure & Function
3. Transformations of Energy

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