Lab 8 Outline

Background Information

• https://openlab.citytech.cuny.edu/bio1101coursehub/lessons/lab/energy/
• https://openlab.citytech.cuny.edu/bio1101coursehub/lessons/lab/enzymes/

Activities

• https://openlab.citytech.cuny.edu/bio1101coursehub/lessons/lab/lab-8-activities/

General Outline

1. Outline the action of enzymes and the corresponding conditions in which they function
   1. Introduce the concept of energy and catalysis (Energy Transformation, Process of Science)
   2. Review nomenclature of enzymes (typically -ase)
   3. Explain the role of guaiacol in the measurement of turnip peroxidase activity
   4. Variables: temperature, concentration, and pH (Structure and Function)
2. Review the quantitative skills regarding independent/dependent variables
   1. Differentiate the variable conditions with the variable of time
   2. Review the idea of rates as a slope of conversion against time (Quantitative Reasoning)
3. Separate the class into four groups that will be responsible for each condition
   1. ENSURE that the groups understand that the reaction begins when ENZYME meets SUBSTRATE
   2. Please have them save the data to a USB flash drive after each condition as a .csv file for later graphing for homework (Quantitative Reasoning)
   3. Have the groups share their data so each student has access to the results of all four independent variable trials

Curricular Notes

These exercises emphasize NSF Vision & Change Core Competency

• Process of Science: emphasis on controls and variables
• Quantitative Reasoning: graphing and interpretation

These exercises emphasize NSF Vision & Change Core Concepts

• Energy Transformation
• Structure and Function

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