Title |
Group 2 Fermentation Tubes, Plates and Data Tables |
Content |
Fermentation Tubes and Plates
Table 1 - Number and Description of Colonies
Table # _____2_______
Names __Yong Wu, Julio Huerta, Jason Bettencourt, Emmanuel Ortiz
Water Analysis Data Collection
Glucose fermentation tubes
Tube ID
(Dilution 1:2)
|
Media Description
(color, turbidity,
sediment, etc)
|
Presence or absence
of gas
|
On a scale of 1 – 5 describe the transparency of the media
|
1 (1:1)
|
Cloudy |
|
2
|
2 (1:2)
|
Clear |
Yes, because there are bubbles in the tube. |
4
|
3 (1:4)
|
Clear |
|
4
|
4 (1:8)
|
Clear |
|
5
|
5 (1:16)
|
Clear |
|
4
|
6 (1: 48)
|
Clear
|
|
5
|
7 ( 1: 144)
|
Clear
|
|
5
|
8 (1:432)
|
|
|
|
Enumeration and Description of Colonies on Nutrient Agar Plates and Membranes
Plate ID
|
Total Number of Colonies
|
Number of Different Colonies
|
Other Observations/Comments
|
Coliforms/100 mL
|
Nutrient agar Plates
|
31
|
7
|
Had some yellow colonies around. Fermentation occurred.
|
310
|
Membrane Filtration
(Dilution 1:2)
|
100
|
5
|
none
|
1000
|
How to calculate the number of coli form per 100 mL of sample
Coliform/100mL = (Number of colonies counted) X 100/(Sample Size, (10) mL) |