Microbiology Lab Fall 2012

 

NA Plate                            Membrane Filter

  

Fermentation Tubes 1        Fermentation tube 2

   

Data Table

Table # __1__________

Names _Leslie, Michelle,Amanda,Scott _

Water Analysis Data Collection

Glucose fermentation tubes

Tube ID

(Dilution 1:2)

Media Description

(color, turbidity, sediment, etc)

Presence or absence of gas

On a scale of 1 – 5 describe the transparency of the media

1 (1:1)

Yellow, clear

2

2 (1:2)

Clear/white, turbid

Presence of gas

5

3 (1:4)

Yellow, clear  

2

4 (1:8)

Yellow, clear  

2

5 (1:16)

Yellow, clear  

2

6 (1: 48)

Yellow, clear  

2

7 ( 1: 144)

Yellow, clear  

2

8 (1:432)

Yellow, clear  

5

 

Enumeration and Description of Colonies on Nutrient Agar Plates and Membranes

Plate ID

Total Number of Colonies

Number of Different Colonies

Other Observations/Comments

 

Coliforms/100 mL

Nutrient agar Plates

43

7

Each colony has a different color and elevation

430

Membrane Filtration

(Dilution 1:2)

65

5

Each colony is not only different by color but shape and size

650

How to calculate the number of coli form per 100 mL of sample

Coliform/100mL = (Number of colonies counted) X 100/(Sample Size, (10) mL)

Leave a Reply

Your email address will not be published.