Figure 1. Methyl red test

Figure 2. Citrate Utilization Test

Figure 3. SIM test

Figure 4. VP test

Figure 5. Litmus milk test

 Discuss the above test and results.

Activity

1.  For each of the figures draw a table and include the observations and results.
2. Write a paragraph on each of the tests given above, giving all relevant information such as the function, the expected observations and results.
3. Using the figures above, give the IMViC results for each bacteria listed.
4. Which of the tests above do/does not require an additional reagent?
5. Which two of the tests above come from the same inoculated test tube?
6. Which of the tests above is/are examining the presence of acid?
7. Which of the tests above is/are involved in examining motility?
8. Which of the above tests can be used to differentiate the following?
   1.  E. coli and P. vulgaris
   2. P. aeruginosa and P. vulgaris
   3. E. coli and E. aerogenes
   4. P. vulgaris and P. aeruginosa
9. Results in Figure 1 and Figure 4 are usually opposite. Why?
10. Name the different pH indicators used in the above tests.