Lab Schedule

 Weeks EXERCISE PAGES
1 1.     Laboratory  Safety. The use and care of the microscope.  Metric system.
2.     Aseptic Techniques.  Inoculation and transfer techniques. Streak plate.		   3, 7,
17, 25
2 3.     Introduction to Staining and Simple Stain.
4.     The Gram stain. Bacterial  anatomy  – discussion  only.		   59
77
 3 5.     The Acid-fast  stain (Ziehl-Neelsen).
6.     Endospore  and capsule  staining.  Negative  staining.		   85, 91,
71,  67
4 7.     Review of Gram Staining and Streak Plate methods. Isolation of pure culture.
8.     The use of Enriched,  Selective  and Differential  media – BA, MS, MC, PEA.
5 9.     Epidemiology  and related topics. Universal  Precautions  (discussion).
10.  Practical  Exam 1.
6 11.  Biochemical  Activities  –  Extracellular  degradation, Hydrolytic  Enzymes.
12.  Carbohydrate  fermentation:  Phenol red broth and Triple Sugar Iron (TSI) agar.		  245-257
267, 273
7 13.  IMViC test.  Multiple  Test Systems:  SIM, Litmus milk,
14.  Urease test, Nitrate reduction  test, Catalase  and Oxidase  test.		 285, 279
295, 301,
149, 155
8 15.  Miniature  Systems – EnteroPluri Test and API. Review of Biochemical tests.
16.  Written Exam 2. The Fungi – Molds, Yeast and Mushrooms.		  231
45
9 17.  The protozoa.
18.  The effect of Temperature  and pH on microorganisms.		   225
10 19.  Atmospheric  Oxygen  Requirements.  Cultivation  of Anaerobes.
20.  The inhibitory  action of heavy metals*.  The inhibitory  action of disinfectants.		  143
11 21.  Antibiotic  susceptibility  testing: The Antibiogram.
22.  Immunology: Discussion  Agglutination, Precipitation and ELISA.		  167
225, 219
12 23.  Written Exam 3. Practical  Exam 2 – Cultivation  of unknowns.
24.  Practical  Exam 2 continues.		 309
13 25.  Practical  Exam 2 concludes.  Pathogenic  Microorganisms  of the Skin*.
26.  Microorganisms  of the Mouth  and Gastrointestinal  Tract.
14 27.  Pathogenic  Microorganisms  of the Respiratory  Tract*.
28.  Pathogenic  Microorganisms  of the Urogenital  System  and STDs
15 29  Helminthology.
30.  Final Written Exam.		    233