Fermentation Tubes and Plates
Table 1 – Number and Description of Colonies
Table # _____2_______
Names __Yong Wu, Julio Huerta, Jason Bettencourt, Emmanuel Ortiz
Water Analysis Data Collection
Glucose fermentation tubes
Tube ID (Dilution 1:2) |
Media Description (color, turbidity, sediment, etc) |
Presence or absence of gas |
On a scale of 1 – 5 describe the transparency of the media |
1 (1:1) |
Cloudy |
2 |
|
2 (1:2) |
Clear | Yes, because there are bubbles in the tube. |
4 |
3 (1:4) |
Clear |
4 |
|
4 (1:8) |
Clear |
5 |
|
5 (1:16) |
Clear |
4 |
|
6 (1: 48) |
Clear
|
5 |
|
7 ( 1: 144) |
Clear |
5 |
|
8 (1:432) |
|
Enumeration and Description of Colonies on Nutrient Agar Plates and Membranes
Plate ID |
Total Number of Colonies |
Number of Different Colonies |
Other Observations/Comments |
Coliforms/100 mL |
Nutrient agar Plates |
31 |
7 |
Had some yellow colonies around. Fermentation occurred. |
310 |
Membrane Filtration (Dilution 1:2) |
100 |
5 |
none |
1000 |
How to calculate the number of coli form per 100 mL of sample
Coliform/100mL = (Number of colonies counted) X 100/(Sample Size, (10) mL)