9. Macromolecules II

Reading

Learning Objectives

  1. Draw the basic structure of an amino acid, and explain the relationships between amino acids, proteins, and peptide bonds.
  2. Describe the levels of organization of protein in terms of its primary, secondary, tertiary, and quaternary structures.
  3. Define the term nucleic acid, give the subunits of a nucleotide, and name the two main types of nucleic acids found in living organisms.
  4. Explain why ATP is important and describe its general structure.

Proteins

AminoAcidballProteins provide much of the structural and functional capacity of cells. Proteins are composed of monomers called amino acids. Amino Acids are hydrocarbons that have an amino group (-NH2) and an acidic carboxyl group (-COOH).The R group represents a hydrocarbon chain with a modification that alters the properties of the amino acid. 20 universal amino acids are used to construct proteins. The variation in functional groups along the amino acid chain gives rise to the functional diversity of proteins.

Amino Acids

20 amino acids and their properties. A 21st amino acid on this table represents the non-universally found selenocysteine.

Monomers bond together through a dehydration synthesis reaction between adjacent amino and carboxyl groups to yield a peptide bond.

peptides

Three amino acids bound into a tripeptide.

How amino acids interact with each other and the environment

Use the following simulation to test how a polypeptide chain with fold based on the type of solution it is in and the composition of the amino acids.

Protein Structure

Main protein structure levels en

  • Primary Structure (1°): The sequence of amino acids read from the Amino or N-terminal end of the molecule to the Carboxyl or C-terminal end
    • Tyr-Cys-Arg-Phe-Leu-Val-….
  • Secondary Structure (2°): local three-dimensional structures that form from interactions of amino acids, like hydrogen bonding
    • Alpha Helix – coils  occurring from the H-bonds between N-H and C=O groups along the backbone of the protein
    • Beta Sheets – laterally connected strands or sheets of amino acids occurring from the H-bonds between N-H and C=O groups along the backbone of the protein
      • Beta sheet bonding antiparallel-colorBeta sheet bonding parallel-color
      • Beta-meander1

        Ribbon diagram of β-sheets

  • Tertiary structure (3°): overall 3-D structure of the peptide chain
  • Quaternary structure(4°): multimeric protein structure from assembling multiple peptide subunits

Diversity of Proteins

Protein structure examples

Credit:Axel Griewel  [CC-BY-SA 3.0]

Learn more about complexity of protein structures at the Protein Data Bank

Protein Function

  1. Enzymes
    • Help “catalyze” chemical reactions
  2. Structural Proteins
    • Give support, strength, flexibility
    • Examples: collagen, elastin, keratin
  3. Storage Proteins
    • Storage of amino acids, for building proteins later or energy
    • Examples: egg albumin (ovalbumin), Casein (in milk)
  4. Transport Proteins
    • Examples: Hemoglobin (O2), H+ pump
  5. Signaling Proteins
    • Hormonal Proteins and receptor proteins
    • Example: Insulin and Insulin receptor
  6. Motor Proteins
    • Actin and myosin perform muscle contraction
  7. Immune Defense Proteins
    • Antibodies help fight infection

Nucleic Acids

Nucleic acids are polymers that were named because they were first described in the nucleus. There are 2 types, deoxyribonucleic acid and ribonucleic acid (DNA & RNA). DNA is the storage vessel for genetic information and RNA largely plays a role in expressing this genetic information.

Their monomers are called nucleotides, which are made up of individual subunits. Nucleotides consist of a 5-Carbon sugar (a pentose), a charged phosphate and a nitrogenous base (Adenine, Guanine, Thymine, Cytosine or Uracil). Each carbon of the pentose has a position designation from 1 through 5. One major difference between DNA and RNA is that DNA contains deoxyribose, and RNA contains ribose. The discriminating feature between these pentoses is at the 2′ position where a hydroxyl group in ribose is substituted with a hydrogen.

DNA chemical structure

DNA has a double helical structure. Two anti-parallel strands are bound by hydrogen bonds.


The following video illustrates the structure and properties of DNA

DNA animation

There are 10 bases for every complete turn in the double helix of DNA.

DNA is a double helical molecule. Two anti-parallel strands are bound together by hydrogen bonds. Adenine forms 2 H-bonds with Thymine. Guanine forms 3 H-bonds with Cytosine. This AT & GC matching is referred to as complementarity. While the nitrogenous bases are found on the interior of the double helix (like rungs on a ladder), the repeating backbone of pentose sugar and phosphate form the backbone of the molecule. Notice that phosphate has a negative charge. This makes DNA and RNA, overall negatively charged.

ATP

  • ATP (adenosine triphosphate) is composed of adenine, ribose, and three phosphates
  • In cells, one phosphate bond is hydrolyzed – Yields:
    • The molecule ADP (adenosine diphosphate)
    • An inorganic phosphate molecule
    • Energy used for work in the cell

ATPanionChemDraw

The structure of ATP. Credit: Smokefoot [CC-BY-SA 4.0]

ADP ATP cycle

Energy is released from the hydrolysis of the terminal phosphate. Credit: Muessig [CC-BY-SA 3.0]

Additional Resources

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