10. Cells

Reading

Learning Objectives

  1. State the components of the cell theory.
  2. Illustrate differences in methods of studying cells
  3. Describe the domains under Prokarya  and differentiate them from Eukarya
  4. Give reasons to explain why cells are generally very small
  5. State the importance of multicellularity.

Slide Show

https://h5p.org/node/273473

Discovery of cells

Hooke’s Cell

Hooke-microscope

Illustration of Hooke’s microscope

In 1665, Robert Hooke published Micrographia, a book that illustrated highly magnified items that included insects and plants. This book spurred on interest in the sciences to examine the microscopic world using lenses but is also notable for Hooke’s observations of cork where he used the word “cell” in a biological sense for the first time.

RobertHookeMicrographia1665

The empty chambers of cork inspired Hooke to coin the term “Cell”

The father of Microbiology: van Leeuwenhoek

Leeuwenhoek Microscope

van Leeuwenhoek’s microscope

The Dutch tradesman Antonie van Leeuwenhoek used high power magnifying lenses to examine the parts of insects and to examine the quality of fabric in his drapery business. He began to experiment with pulling glass to generate lenses and developed a simple microscope to observe samples. Using a simple single lens with a specimen mounted on a point, he was able to identify the first microscopic “animalcules” (little animals) that will be later known as protozoa (original animals).

Though van Leeuwenhoek’s apparatus was simple, the magnifying power of his lenses and his curiosity enabled him to  perform great scientific observations on the the microscopic world. He was ridiculed for fabricating his observations of protists at first. Ever the scientist, van Leeuwenhoek examined samples of his own diarrhea  to discover Giardia intestinalis. While he did not make the connection of the causative nature of this microorganism, he described the details of the way this organism could propel itself through the medium in great detail.

van Leeuwenhoek also visualized sperm cells from animals and observed the process of fertilization of egg cells. This observation was extremely important because it negated the idea of spontaneous generation, the notion that life can spring forth from inanimate material. Spontaneous generation previously explained the observations that maggots could arise from decaying meat and fleas arose from dust.

Giardia intestinalis (259 17)

Modern micrograph of Giardia intestinalis (Kingdom Protista) Credit: Doc. RNDr. Josef Reischig, CSc. [CC-BY-SA 3.0]

Life arises from life

Plague Doctor

The plague doctor wore a mask to protect from “bad air”. CC-BY-SA Matankic

The discovery of the microscopic world enlightened scientists and aided in abolishing spontaneous generation and furthered invented the field of microbiology. Prior to this, a popular idea for spread of diseases like bubonic plague involved the spread of miasma (“bad air”). To protect himself from the bad air, the plague doctor wore a beaked mask filled with fragrant items  and a built in respirator.

Pasteur, fermentation and Germ theory of disease

Louis Pasteur Experiment

Pasteur’s experiments on spontaneous generation Credit: Kgerow16 [CC-BY 4.0]

Pasteur conducted experiments with the fermentation of nutrient broth in goose-necked flasks. Upon the discovery of the microscopic world, it was thought that microbes could be spread. Goose-necked flasks could be boiled to sterilize the nutrients while protecting the contents from contacting external microbes. the removal of the goose-neck or movement of broth into the goose-neck resulted in the subsequent fermentation of nutrient broth while the intact flasks remained sterile.

Pasteur's experiment testing spontaneous generation and biogenesis

Animated version of Pasteur’s experiments on spontaneous generation Credit: Thebiologyprimer [CC-BY 4.0]

Modern Compound Microscope

Unlike van Leeuwenhoek’s single lens microscope, we now combine the magnifying power of multiple lenses in what is called a compound microscope.

Compound Light Microscope

Compound Light Microscope. Credit: jeremy Seto [CC-BY-NC-SA]

Electron Microscopy

An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination.  A scanning transmission electron microscope has magnifications of up to about 10,000,000x whereas most light microscopes have a limit of magnifications below 2000x

Transmission Electron Microsopy

Scheme TEM en

Schematic of Transmitting Electron Microscope. Credit: Gringer [CC-BY-SA 3.0]

Scanning Electron Microscopy

Misc pollen

Pollen under a scanning electron microscope. [CC0]

Fluorescence Microscopy

Fluorescence Microscope

Fluorescent Microscope. Credit: jeremy Seto [CC-BY-NC-SA]

FluorescenceFilters 2008-09-28

Schematic of a fluorescent micrscope. Credit: Henry Mühlpfordt [CC-BY-SA 3.0]

https://upload.wikimedia.org/wikipedia/commons/a/a6/Fluorescent_and_confocal_microscopes.ogv

GFP in HEK293T

Light micrograph overlayed with a fluorescent micrograph of green fluorescent protein expressing cells. Credit: jeremy Seto [CC-BY-NC-SA]

Dissecting Microscope

Dissecting Microscope

Dissecting Microscope. Credit: jeremy Seto [CC-BY-NC-SA]

Prokaryotes

Anillo de la vida

Ring of life diagram illustrating the divergence of prokaryotes and the subsequent endosymbiosis that gave rise to eukaryotes. Credit: Maulucioni [CC-BY-SA 3.0]

Prokaryote cell

Schematic diagram of a prokaryotic cell. Credit: Ali Zifan [CC-BY-SA 4.0]

Bacterial morphology diagram

Basic shapes of bacteria. [CC0]