Biotechnology

RNA-Seq

RT-PCR and RT-qPCR can be used to measure the abundance of specific transcripts in a fairly low throughput way. Leveraging the the concept of Reverse Transcription and coupling that to high-throughput sequencing technologies, transcripts can be sequenced and mapped to a genome to depict the quantity of transcripts as represented by number of reads.

Given sufficient read coverage, novel splice isoforms can also be identified as different exon-exon junctions are identified.

 

The general workflow of RNA-Seq analysis follows: