Fermentation Tubes Agar Plate Membrane Filter
Table # 5
Names: Bianca, Leonie, Crystal, Dorothy, Jamie-lee
Water Analysis Data Collection
Glucose fermentation tubes
|
Tube ID (Dilution 1:2) |
Media Description (color, turbidity, sediment, etc) |
Presence or absence of gas |
On a scale of 1 – 5 describe the transparency of the media |
|
1 (1:1) |
Yellow w/ sediments | 5 | |
|
2 (1:2) |
Yellow w/ sediments | Yes | 5 |
|
3 (1:4) |
Yellow w/sediments and fragmented | 5 | |
|
4 (1:8) |
Yellow | 5 | |
|
5 (1:16) |
Yellow w/ sediments and fragmented | 1 | |
|
6 (1: 48) |
Orange, cloudy,fragments | 1 | |
|
7 ( 1: 144) |
Red, clear, sediments | 5 | |
|
8 (1:432) |
Red, clear, sediments | 5 |
Enumeration and Description of Colonies on Nutrient Agar Plates and Membranes
|
Plate ID |
Total Number of Colonies |
Number of Different Colonies |
Other Observations/Comments |
Coliforms/100 mL |
|
Nutrient agar Plates |
23 |
5
|
Some elevation and conex |
None |
|
Membrane Filtration (Dilution 1:2) |
18 |
3 |
Some blue, some yellow, some red, some big, and some small |
None |
How to calculate the number of coli form per 100 mL of sample
Coliform/100mL = (Number of colonies counted) X 100/(Sample Size, (10) mL)
Under the microscope!!!!!
C3 Test-tubes
C6 Test-tubes
Argar Plates
PEA Plates
MacConkey Plates
Blood Agar Plates
Results
|
Unknown Organism ID |
Grain Stain Reaction |
Growth on PEA Agar |
Growth on MacConkey Agar |
Growth on Blood Agar |
Comments/ Observations |
| C1 | RodGram + | Growth | Growth | Gamma | |
| C2 | Growth | None | |||
| C3 | RodGram – | Growth | Growth | Alpha | |
| C4 | RodGram+ | Growth | None | Alpha | |
| C5 | RodGram + | Growth | None | Alpha | |
| C6 | Rod, Gram + | Growth | None | Alpha | |
| C7 | CocciGram – | Growth | Growth | Alpha | |
| C8 | RodGram + | Growth | Growth | Beta |
| Organism | Indole SIMS | Methyol Red | VP | Citrate | H₂S | Motility | PR-Lactose | PR-Sucrose | PR-Glucose | PR-Mannitol | PR-Mulin | Bile Esculin | Urea | Nitrate Reduction | Catalase | Oxidase | Oxygen | Mannitol |
| C1 | Negative | Negative | Negative | Negative (no black butt) | None | Orange/Gas | Orange/Gas | Orange(red)/No Gas | Positive/Negative | Negative | Positive | Negative | Positive | Positive | Positive | Growth(yellow) | ||
| C3 | Positive | Negative | Negative | Negative (green) | Negative (no black butt) | None | Red/No gas | Yellow | Yellow/No Gas | Positive | Positive (hot pink)/ No gas/Cloudy | Negative | Negative | Positive | Negative | Positive | No growth | |
| C4 | Negative | Negative | Negative | Negative (no black butt) | None | Red/Gas | Red/Gas | Yellow/No Gas | Negative | Negative/Cloudy | Positive | Negative | Positive | Positive | Positive | No growth | ||
| C5 | Negative | Negative | Negative | Negative (no black butt) | None | Yellow/No gas | Red/Gas | Yellow/No Gas | Negative | Negative/Cloudy/Sediments | Negative | Negative | Positive/Cloudy | Positive | Positive | No growth | ||
| C6 | Negative | Negative | Positive (red) | Negative (green) | Negative (no black butt) | None | Red/No gas | Yellow | Yellow/No Gas | Positive | Positive (hot pink)/ No gas | Positive | Negative | Negative/Cloudy | Positive | Positive | Growth | |
| C7 | Negative | Negative | Positive (red) | Negative (no black butt) | None | Red/No gas | Yellow/Gas | Yellow/No Gas | Positive/Negative | Negative | Positive | Negative | Positive | Positive | Positive | Growth | ||
| C7#2 | Negative | Positive | Positive (red) | Negative (no black butt) | None | Red/No gas | Orange/Gas | Yellow/No Gas | Positive/Negative | Negative | Positive | Negative | Positive | Positive | Positive | Growth | ||
| C8 | Negative | Negative | Positive (red) | Negative (no black butt) | None | Red/No gas | Yellow/No Gas | Orange(red)/No Gas | Positive/Negative | Negative | Positive | Negative | Negative | Positive | Positive | Growth |
