Microbiology Lab Fall 2012

 

 

 

 

Fermentation Tubes                     Agar Plate                                 Membrane Filter

Group 5 Fermentation Tube Set 1 Group 5 Agar Plate   Group 5 Membrane Filter

 

 

Table # 5

Names: Bianca, Leonie, Crystal, Dorothy, Jamie-lee

Water Analysis Data Collection

Glucose fermentation tubes

Tube ID

(Dilution 1:2)

Media Description

(color, turbidity, sediment, etc)

Presence or absence of gas

On a scale of 1 – 5 describe the transparency   of the media

1 (1:1)

Yellow w/ sediments 5

2 (1:2)

Yellow w/ sediments Yes 5

3 (1:4)

Yellow w/sediments and fragmented 5

4 (1:8)

Yellow 5

5 (1:16)

Yellow w/ sediments and fragmented 1

6 (1: 48)

Orange, cloudy,fragments 1

7 ( 1: 144)

Red, clear, sediments 5

8 (1:432)

Red, clear, sediments 5

 

Enumeration and Description of Colonies on Nutrient Agar Plates and Membranes

Plate ID

Total Number of Colonies

Number of Different Colonies

Other Observations/Comments

 

Coliforms/100 mL

Nutrient agar Plates

23

5

Some elevation and conex

None

Membrane Filtration

(Dilution 1:2)

18

3

Some blue, some yellow, some red, some   big, and some small

None

How to calculate the number of coli form per 100 mL of sample

Coliform/100mL = (Number of colonies counted) X 100/(Sample Size, (10) mL)

 

Under the microscope!!!!!

https://openlab.citytech.cuny.edu/wp-content/uploads/group-documents/1264/1355597387-c1.jpg

https://openlab.citytech.cuny.edu/wp-content/uploads/group-documents/1264/1355597450-c3.jpg

https://openlab.citytech.cuny.edu/wp-content/uploads/group-documents/1264/1355597658-c5.jpg

https://openlab.citytech.cuny.edu/wp-content/uploads/group-documents/1264/1355597784-c8a.jpg

 

 

C3 Test-tubes

C6 Test-tubes

Argar Plates

     

 

PEA Plates

https://openlab.citytech.cuny.edu/wp-content/uploads/group-documents/1264/1355594680-20121129_102846.jpg

 

MacConkey Plates

https://openlab.citytech.cuny.edu/wp-content/uploads/group-documents/1264/1355595498-20121129_102955.jpg

 

Blood Agar Plates

https://openlab.citytech.cuny.edu/wp-content/uploads/group-documents/1264/1355596289-20121129_103156.jpg

 

 

Results

Unknown Organism ID

Grain Stain Reaction

Growth on PEA Agar

Growth on MacConkey Agar

Growth on Blood Agar

Comments/ Observations

C1 RodGram + Growth Growth Gamma
C2 Growth None
C3 RodGram – Growth Growth Alpha
C4 RodGram+ Growth None Alpha
C5 RodGram + Growth None Alpha
C6 Rod, Gram + Growth None Alpha
C7 CocciGram – Growth Growth Alpha
C8 RodGram + Growth Growth Beta

 

 

Organism Indole SIMS Methyol Red VP Citrate H₂S Motility PR-Lactose PR-Sucrose PR-Glucose PR-Mannitol PR-Mulin Bile Esculin Urea Nitrate Reduction Catalase Oxidase Oxygen Mannitol
C1 Negative Negative Negative Negative (no black butt) None Orange/Gas Orange/Gas Orange(red)/No Gas Positive/Negative Negative Positive Negative Positive Positive Positive Growth(yellow)
C3 Positive Negative Negative Negative (green) Negative (no black butt) None Red/No gas Yellow Yellow/No Gas Positive Positive (hot pink)/ No gas/Cloudy Negative Negative Positive Negative Positive No growth
C4 Negative Negative Negative Negative (no black butt) None Red/Gas Red/Gas Yellow/No Gas Negative Negative/Cloudy Positive Negative Positive Positive Positive No growth
C5 Negative Negative Negative Negative (no black butt) None Yellow/No gas Red/Gas Yellow/No Gas Negative Negative/Cloudy/Sediments Negative Negative Positive/Cloudy Positive Positive No growth
C6 Negative Negative Positive (red) Negative (green) Negative (no black butt) None Red/No gas Yellow Yellow/No Gas Positive Positive (hot pink)/ No gas Positive Negative Negative/Cloudy Positive Positive Growth
C7 Negative Negative Positive (red) Negative (no black butt) None Red/No gas Yellow/Gas Yellow/No Gas Positive/Negative Negative Positive Negative Positive Positive Positive Growth
C7#2 Negative Positive Positive (red) Negative (no black butt) None Red/No gas Orange/Gas Yellow/No Gas Positive/Negative Negative Positive Negative Positive Positive Positive Growth
C8 Negative Negative Positive (red) Negative (no black butt) None Red/No gas Yellow/No Gas Orange(red)/No Gas Positive/Negative Negative Positive Negative Negative Positive Positive Growth

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