Real biological examples

We can see the concepts of FOV and DOF in the following pictures.
Rosie on a fence post
Close-up of Rosie on a fence post
In an even more extreme close-up (higher magnification), we would have difficulty focusing on both the eyes and beak since there is depth and distance between those features.

How do we use microscopes

In our lab, we look at some pond water. What do we see? Why is this significant? How does the microscope help us study these items? What is the utility of the the concepts of magnification, FOV and DOF when we use microscopes to study biological samples?

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