Contents
Effect of Enzyme Concentration on Enzyme Activity
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- Add 5 ml of water to an empty tube (this is the BLANK)
- Add 5 ml of pH 7 buffer to 3 separate tubes
- Follow the dilution scheme below:
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- In 4 separate tubes, ADD 4 ml of starch solution
- label them 1x, 1/5x, 1/25x, 1/125x
- Add 2 drops of iodine to each starch tube and the Blank
- Read the Blank in the spectrophotometer and calibrate it to 100% transmittance at 560nm
- Read each tube in the spectrophotometer. This is time 0 min
- Add 35 drops of diluted amylase solutions to the appropriately labeld tubes simultaneously and mix.
- Each tube receives a different Amylase dilution
- At 2 minute intervals, quickly read ALL tubes in the spectrophotometer.
- Continue reading the samples every 2 minutes until you reach 22 minutes on the table below.
- In 4 separate tubes, ADD 4 ml of starch solution
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Time
(min)
1X
% Trans
1/5X
% Trans
1/25X
% Trans
1/125X
% Trans
0
2
4
6
8
10
12
14
16
18
20
22
Tags: integration of knowledge, quantitative reasoning, analysis, inquiry