NA Plate Membrane Filter
Fermentation Tubes 1 Fermentation tube 2
Data Table
Table # __1__________
Names _Leslie, Michelle,Amanda,Scott _
Water Analysis Data Collection
Glucose fermentation tubes
|
Tube ID (Dilution 1:2) |
Media Description (color, turbidity, sediment, etc) |
Presence or absence of gas |
On a scale of 1 – 5 describe the transparency of the media |
|
1 (1:1) |
Yellow, clear |
|
2 |
|
2 (1:2) |
Clear/white, turbid |
Presence of gas |
5 |
|
3 (1:4) |
Yellow, clear |
2 |
|
|
4 (1:8) |
Yellow, clear |
2 |
|
|
5 (1:16) |
Yellow, clear |
2 |
|
|
6 (1: 48) |
Yellow, clear |
2 |
|
|
7 ( 1: 144) |
Yellow, clear |
2 |
|
|
8 (1:432) |
Yellow, clear |
5 |
Enumeration and Description of Colonies on Nutrient Agar Plates and Membranes
|
Plate ID |
Total Number of Colonies |
Number of Different Colonies |
Other Observations/Comments |
Coliforms/100 mL |
|
Nutrient agar Plates |
43 |
7
|
Each colony has a different color and elevation |
430 |
|
Membrane Filtration (Dilution 1:2)
|
65 |
5 |
Each colony is not only different by color but shape and size |
650 |
How to calculate the number of coli form per 100 mL of sample
Coliform/100mL = (Number of colonies counted) X 100/(Sample Size, (10) mL)
